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There are four CaM molecules per RyR channel, i. For luciferase and β-galactosidase assays, add 100 μL lysis buffer per well of a 12-well plate, incubate 20 min at room temperature, do NOT shake. The device is then maintained for another 3 months before its removal. Lluch, the contact will close and the circuit will allow M2 to be energized, if the reverse pushbutton is pressed.

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